BRK (Phospho-Y342) Polyclonal Antibody-BS64552

BRK (Phospho-Y342) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS64552-50, BS64552-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13882

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC

All Applications: WB:1:500~1:1000 IHC:1:50~1:200

Background: The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: BRK (Phospho-Y342) polyclonal antibody detects endogenous levels of BRK protein only when phosphorylated at Tyr342.

Bioworld Molecular Weight: ~ 52 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot (WB) analysis of BRK (Phospho-Y342) polyclonal antibody at 1:500 dilution
Lane A: HEK293T whole cell lysate
Lane B: HCT116 whole cell lysate
Lane C: NIH3T3 whole cell lysate

Alternative Name: Protein-tyrosine kinase 6; Breast tumor kinase; Tyrosine-protein kinase BRK; PTK6; BRK

Immunogen: Synthetic phosphopeptide derived from human BRK around the phosphorylation site of Tyrosine 342.

Conjugate: Unconjugated

Modification: Phosphorylation