Product Description

Brn-3c Polyclonal Antibody

Catalogue Numbers: BS5632-50, BS5632-100

Sizes: 50µl, 100µl

Alternative Name: POU domain, class 4, transcription factor 3; Brain-specific homeobox/POU domain protein 3C; Brain-3C; Brn-3C; POU4F3; BRN3C

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15319

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Three Brn-3 proteins have been described and are designated Brn-3a, Brn-3b and Brn-3c. Brn-3a has two functional transactivating domains, one at the amino terminus and one at the carboxy terminus. While Brn-3a and Brn-3c stimulate transcription, Brn-3b generally functions as a transcriptional repressor. However, Brn-3b, but not Brn-3a, has been shown to regulate the expression of the acetylcholine receptor.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Brn-3c polyclonal antibody detects endogenous levels of Brn-3c protein.

Bioworld Molecular Weight: ~ 37 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Brn-3c polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 263-308 of Human Brn-3c.

Conjugate: Unconjugated

Modification: Unmodified