BTF3L4 Polyclonal Antibody
Catalogue Numbers: BS60514-50, BS60514-100
Sizes: 50µl, 100µl
Alternative Name: Transcription factor BTF3 homolog 4; Basic transcription factor 3-like 4; BTF3L4
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q96K17
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: The initiation of gene transcription involves the ordered assembly of a multiprotein complex on proximal promoter elements such as the TATA box. In addition to RNA polymerase II (Pol II), the transcription factor class II (TFII) family of proteins are required for initiation of transcription, as the first step in the formation of this initiation complex is the stable binding of TFIID to the TATA box. An additional TFII-related protein, BTF3, does not directly associate with the proximal promoter, but rather forms a stable complex with Pol II and facilitates Pol II assembly into the complex. The BTF3 gene is ubiquitously expressed and encodes two protein isoforms, BTF3a and BTF3b, which are produced from alternative splicing. The BTF3 proteins are identical, except that BTF3b lacks the first 44 amino acids at the N-terminal of BTF3a. As a consequence of this deletion, BTF3b is unable to induce transcription, despite being able to bind Pol II. Additionally, BTF3a and BTF3b associate with the widely expressed protein kinase casein kinase II. Casein kinase II phosphorylates BTF3a, as well as TFIIB, and is required for the efficient transcription of the tRNA and 55 rRNA genes by Pol III. BTF3 belongs to the NAC-β family, which includes several related proteins, such as BTF3L1, BTF3L2, BTF3L3 and BTF3L4.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: BTF3L4 polyclonal antibody detects endogenous levels of BTF3L4 protein.
Bioworld Molecular Weight: ~ 17 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of BTF3L4 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: PC12 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human BTF3L4
Conjugate: Unconjugated
Modification: Unmodified