Product Description

BTK Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66519-50, MB66519-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Bruton's tyrosine kinase (Btk) is a member of the Btk/Tec family of cytoplasmic tyrosine kinases. Like other Btk family members, it contains a pleckstrin homology (PH) domain and Src homology SH3 and SH2 domains. Btk plays an important role in B cell development . Activation of B cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3, 4, 5-trisphosphate . The membrane-localized Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinases, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for fμll activation . The activation of Btk is negatively regμlated by PKCβ through phosphorylation of Btk at Ser180, which resμlts in reduced membrane recruitment, transphosphorylation, and subsequent activation. The PKC inhibitory signal is likely to be a key determinant of the B cell receptor signaling threshold to maintain optimal Btk activity.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IP

Alternate Names: AGMX1, ATK, BPK, Tyrosine-protein kinase BTK, Agammaglobμlinemia tyrosine kinase, ATK, B-cell progenitor kinase, BPK, Bruton tyrosine kinase

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Purified recombinant human BTK protein fragments expressed in E.coli.

Dilution: WB (1/500 - 1/1000), IP (1/10 - 1/50)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of BTK protein.

Molecular Weight: ~ 77 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q06187

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only