BTK (phospho-Y223) Rabbit Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS9948M-50, BS9948M-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q06187
Host: Rabbit
Reactivity: Human
Applications: WB
All Applications: WB: 1:1000-1:2000
Background: Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases. Like other BTK family members, it contains a pleckstrin homology (PH) domain, Src homology SH3 and SH2 domains. BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation. The activation of BTK is negatively regulated by PKC beta through phosphorylation of BTK at Ser180, which results in reduced membrane recruitment, transphosphorylation and subsequent activation.
Purification and Purity: Protein A affinity purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of BTK (phospho-Y223) Rabbit mAb at 1:1000 dilution
Lane 1:K562 whole cell lysate treated with pervanadate
Lane 2:K562 whole cell lysate
Bioworld Molecular Weight: ~ 77 kDa
Specificity: This antibody detects endogenous levels of BTK protein only when phosphorylated at Tyr223.
Alternative Name: Tyrosine-protein kinase BTK; Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase; BTK; AGMX1; ATK; BPK
Immunogen: Recombinant antibody.
Conjugate: Unconjugated
Modification: Phosphorylation