BUB3 (H274) Polyclonal Antibody-BS2395

BUB3 (H274) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2395-50, BS2395-100

Sizes: 50µl, 100µl

Swiss-Prot: O43684

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: BUB3 (budding uninhibited by benzimidazoles 3 homolog), also known as BUB3L or hBUB3, is a conserved component of the mitotic spindle assembly complex (MCC). It contains five WD repeat domains and forms cell cycle constitutive complexes with BUB1 and BUBR1. BUB3 is essential for the kinetochore localization of BUB1 and BUBR1. As a component of the MCC, BUB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. The spindle checkpoint pathway functions to postpone the initiation of anaphase until chromosomes are properly attached to the spindle. This acts to ensure accurate chromosome segregation. In addition, BUB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments. BUB3 is also thought to bind Tctex1L (or DYNLT3), a dynein light chain.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: BUB3 (H274) polyclonal antibody detects endogenous levels of BUB3 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Bub3 (H274) pAb at 1:500 dilution
Lane 1: A549 whole cell lysate (40ug)
Lane 2: HEK293T whole cell lysate (40ug)
Lane 3: PC12 whole cell lysate (40ug)
Lane 4: 3T3-L1 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of BUB3 (H274) pAb in paraffin-embedded human brain tissue.

Bioworld Molecular Weight: ~ 40 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: BUB3; BUB3L; HBUB3; Mitotic checkpoint protein BUB3

Immunogen: Synthetic peptide, corresponding to amino acids 251-300 of Human BUB3.

Conjugate: Unconjugated

Modification: Unmodified