Product Description

BUBR1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11941-50, MB11941-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: O60566

Host: Rabbit

Reactivity: Human, Hamster

Applications: WB, IHC-P, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20

Background: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 120 kDa; Observed MW: 120 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of BubR1 in CHO-K1, Ramos lysates using BUBR1 antibody., Immunohistochemistry analysis of paraffin-embedded Human lung cancer using BubR1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: BUB1B; BUBR1; MAD3L; SSK1; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L; Protein SSK1

Immunogen: A synthetic peptide of human BubR1

Conjugate: Unconjugated

Modification: Unmodified