Product Description

BubR1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90152-50, BS90152-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O60566(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC IHC FC

All Applications: WB:1:5,000-1:10,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Human cells contain two related protein kinases, BUB1 and BUBR1, that appear to have evolved from a single ancestral BUB1 gene. Both kinases are concentrated near the surface of the kinetochore where they monitor kinetochore-microtubule interactions. BUB1 and BUBR1 bind to kinetochores and are postulated to be components of the mitotic checkpoint, which monitors kinetochore activities to determine if chromosomes have achieved alignment at the spindle equator. BUBR1 is essential for normal mitotic progression as it prevents cells from prematurely entering anaphase. BUB3 is a conserved component of the mitotic spindle assembly complex and is also involved with the essential spindle checkpoint pathway that operates during early embryogenesis.

Purification and Purity: Protein affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: BubR1 polyclonal antibody detects endogenous levels of BubR1 protein.

Bioworld Molecular Weight: 119 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of BubR1 on SH-SY-5Y cell lysate using anti-BubR1 antibody at 1/10,000 dilution., ICC staining BubR1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: hBUBR1, BUB1B, BUBR1, MAD3L, SSK1, Beta homolg of S. cerevisiae BUB 1, Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles, BUB 1B, BUB1 budding uninhibited by benzimidazoles 1 homolog beta, Bub1A, BUB1B, BUB1B, BUB1beta, BUBR1, Budding Uninhibited by Benzimidazoles 1 beta, Budding uninhibited by benzimidazoles 1 homolog beta (yeast), hBUBR1, MAD3/BUB1 related protein kinase, MAD3/BUB1-related protein kinase, MAD3L, Mitotic checkpoint gene BUB1B, Mitotic checkpoint kinase MAD3L, Mitotic checkpoint serine/threonine protein kinase BUB1 beta, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, MVA1, OTTHUMP00000160319, Protein SSK1, SSK 1, SSK1,

Immunogen: Recombinant protein within human BubR1 330-600 aa.

Conjugate: Unconjugated

Modification: Unmodified