BUBR1 Polyclonal Antibody
Product: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sizes: 50µl, 100µl
Catalogue Numbers: BZ16811-50, BZ16811-100
Swiss-Prot: O60566
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IP, FC
All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20 FC: 1/50-1/100
Background: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Calculated MW: 120 kDa; Observed MW: 120 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of BubR1 in HepG2 lysates using BUBR1 antibody.
Alternative Name: BUB1B; BUBR1; MAD3L; SSK1; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L; Protein SSK1
Immunogen: A synthesized peptide derived from human BubR1
Conjugate: Unconjugated
Modification: Unmodified