C/EBP-β (phospho-T235/188) Polyclonal Antibody-BS4029

C/EBP-β (phospho-T235/188) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS4029-50, BS4029-100

Swiss-Prot: P17676

Host: Rabbit

C/EBP-β (phospho-T235/188) Polyclonal Antibody-BS4029

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: C/EBP β is a member of the C/EBP transcription factor family. The C/EBP β gene encodes several isoforms, which have truncated transcription activation domains by the alternative translational initiation at multipe AUG start sites. Initiation of translation at the in-frame AUGs forms four C/EBP β isoforms. C/EBP β is also known as interleukin 6-dependent DNA-binding protein (IL6DBP), liver activator protein(LAP) or liver-enriched transcriptional activator protein transcription factor 5 (TCF5). C/EBP β contributes to the regulation of the acute phase response in hepatocytes. Stat3 has an important function in IL-6-mediated transcription of the C/EBP β gene that has direct implication for acute phase response in liver cells.The 35 kDa C/EBP β form requires phosphorylation for its DNA binding ability, and increased binding of C/EBP β isoforms during acute-phase reaction occurs through its upregulation and structural modification.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-C/EBP-β (T235/188) polyclonal antibody detects endogenous levels of human LAP only when phosphorylated at Thr235, mouse and rat LAP only when phosphorylated at Thr188, and LIP only when phosphorylated at Thr37.

Bioworld Molecular Weight: ~ 38 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of C/EBP-β (phospho-T235/188) pAb at 1:500 dilution
Lane 1: MCF-7 treated with insulin (500ng/ml,5min) whole cell lysate (40ug)
Lane 2: MCF-7 treated with insulin (500ng/ml,0min) whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of p-C/EBP-β (T235/188) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Alternative Name: CCAAT/enhancer-binding protein beta; C/EBP β; C/EBPβ; C/EBP beta; Liver activator protein; LAP; Liver-enriched inhibitory protein; LIP; Nuclear factor NF-IL6; Transcription factor 5; TCF-5; CEBPB; TCF5; ORF Names: PP9092

Immunogen: Synthetic phosphopeptide derived from human C/EBP-β around the phosphorylation site of Threonine 235.

Modification: Phosphorylation

Conjugate: Unconjugated