C/EBP-γ (E64) Polyclonal Antibody-BS2558

C/EBP-γ (E64) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2558-50, BS2558-100

Sizes: 50µl, 100µl

Swiss-Prot: P53567

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: The transcription factor C/EBP α (CCAAT-enhancer binding protein) is a heatstable, sequence-specific DNA-binding protein first purified from rat liver nuclei that binds avidly to several different cis-regulatory DNA sequences commonly associated with viral and cellular genes transcribed by RNA polymerase II. C/EBP α regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP α uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP α appears to function exclusively in terminally differentiated, growth-arrested cells. Additional family members include C/EBP β, C/EBP γ,C/EBP δ and C/EBP ε, all of which exhibit similar DNA-binding specificities and affinities to C/EBP α. Furthermore, C/EBP β and C/EBP δ readily form heterodimers both with each other as well as with C/EBP α.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: C/EBP-γ (E64) detects endogenous levels of C/EBP-γ protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of C/EBP-γ (E64) polyclonal antibody at 1:500 dilution
Lane 1: SGC-7901 whole cell lysate (40ug)
Lane 2: The testis tissue lysate of Mouse (30ug), Immunohistochemistry (IHC) analyzes of C/EBP-γ (E64) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 22 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: CCAAT/enhancer-binding protein gamma; C/EBP gamma; CEBPG; C/EBPγ; CEBPγ

Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human C/EBP-γ.

Conjugate: Unconjugated

Modification: Unmodified