C/EBP epsilon (Phospho-T74) Polyclonal Antibody-BS64519

C/EBP epsilon (Phospho-T74) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS64519-50, BS64519-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15744

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200

Background: C/EBP α regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP α uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic α helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP α appears to function exclusively in terminally-differentiated, growth-arrested cells. Additional family members include C/EBP β, C/EBP γ, C/EBP δ and C/EBP ε, all of which exhibit similar DNA-binding specificities and affinities to C/EBP α. Furthermore, C/EBP β and C/EBP δ readily form heterodimers both with each other as well as with C/EBP α.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: C/EBP epsilon (Phospho-T74) polyclonal antibody detects endogenous levels of C/EBP epsilon protein only when phosphorylated at Thr74.

Bioworld Molecular Weight: ~ 34 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of C/EBP epsilon (Phospho-T74) polyclonal antibody at 1:500 dilution
Lane A: Hela whole cell lysate
Lane B: The Liver tissue lysate of Rat
Lane C: Raw264.7 whole cell lysate
Lane D: The Liver tissue lysate of Rat

Alternative Name: CCAAT/enhancer-binding protein epsilon; C/EBP epsilon; CEBPE; C/EBPε

Immunogen: Synthetic phosphopeptide derived from human C/EBP epsilon around the phosphorylation site of Threonine 74.

Conjugate: Unconjugated

Modification: Phosphorylation