c-Fos Polyclonal Antibody-BS90295

c-Fos Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS90295-50, BS90295-100

Swiss-Prot: P01100

Host: Rabbit

c-Fos Polyclonal Antibody-BS90295

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 55-60KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of HeLa cells, using c-Fos antibody at 1:1000 dilution.HeLa cells were treated by PMA/TPA at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunofluorescence analysis of NIH/3T3 cells using c-Fos Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: FOS; AP-1; C-FOS; p55; c-Fos

Immunogen: Recombinant fusion protein of human c-Fos(NP_005243.1).

Modification: Unmodified

Conjugate: Unconjugated