c-Jun Polyclonal Antibody-BS90305

c-Jun Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90305-50, BS90305-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P05412(Human) P05627(Mouse) P17325(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC, IHC, IP,

All Applications: WB:1:1,000
ICC:1:100-1:500
IHC:1:50-1:200

Background: Genes belonging to the Jun and Fos oncogene families encode nuclear proteins that are associated with a number of transcriptional complexes. The c-Jun protein is a major component of the transcription factor AP-1, originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA-response element (TRE). The Jun proteins form homo- and heterodimers which bind the TRE, while Fos proteins are active only as heterodimers with any of the Jun proteins. Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain. An inhibitor of Fos/Jun function, termed IP-1, associates with Fos and Jun and is inactivated upon phosphorylation induced by the cAMP-dependent protein kinase A (PKA).

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: c-Jun polyclonal antibody detects endogenous levels of c-Jun protein.

Bioworld Molecular Weight: 43 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining c-Jun in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining c-Jun in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: AP1, JUN, Activator protein 1, AP 1, AP1, cJun, Enhancer Binding Protein AP1, Jun Activation Domain Binding Protein, JUN, Jun oncogene, JUN protein, Jun proto oncogene, JUN, JUNC, Oncogene JUN, p39, Proto oncogene c jun, Proto oncogene cJun, Proto-oncogene c-jun, Transcription Factor AP 1, Transcription factor AP-1, Transcription Factor AP1, V jun avian sarcoma virus 17 oncogene homolog, V jun sarcoma virus 17 oncogene homolog (avian), V jun sarcoma virus 17 oncogene homolog, V-jun avian sarcoma virus 17 oncogene homolog, vJun Avian Sarcoma Virus 17 Oncogene Homolog,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified