c-Myb (Phospho-S11) Polyclonal Antibody-BS94005

c-Myb (Phospho-S11) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS94005-50, BS94005-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P10242(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: The Myb family of transcription factors regulates proliferation, differentiation and apoptosis of hematopoletic cells. The avian myeloblastosis viral (v-Myb) protein is nuclear and binds to specific DNA sequences. The gene encoding for v-Myb is oncogenic, causing monoblastic leukemia and transforming myelomonocytic cells by deregulating the expression of specific target genes. v-Myb functions as a transcriptional activator, and it can repress biologically relevant genes such as Ets-2, which promotes macrophage differentiation. The proto-oncogene c-Myb encodes for a nuclear protein that plays a role in transcriptional regulation and may be essential for hematopoietic cell proliferation. A-Myb is also member of the Myb family that is expressed in proliferating B cell centroblasts. Transgenic mice overexpressing A-Myb posses enhanced hyperplasia of the lymph nodes.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: c-Myb (Phospho-S11) polyclonal antibody detects endogenous levels of c-Myb protein only when phosphorylated at S11.

Bioworld Molecular Weight: 72/75 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining P-V-Myb+C-Myb(S11) in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., Immunohistochemical analysis of paraffin-embedded human uterine neck tissue using anti-P-V-Myb+C-Myb(S11) antibody. Counter stained with hematoxylin.

Alternative Name: MYB, C myb, C myb protein, Cmyb, MYB, Myb proto oncogene, V myb myeloblastosis viral oncogene homolog,

Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser11 of human V-Myb+C-Myb.

Conjugate: Unconjugated

Modification: Phosphorylation