c-Myc (Phospho-S62) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS94081-50, BS94081-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P01106(Human) P01108(Mouse) P09416(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, IP,
All Applications: WB:1:1,000-1:2,000
IHC:1:50-1:200
ICC:1:50-1:200
Background: c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein, designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with at least two additional bHLH-Zip proteins, Mad and Mxi1, and Mad-Max dimers have been shown to repress transcription through interaction with mSin3.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: c-Myc (Phospho-S62) polyclonal antibody detects endogenous levels of c-Myc protein only when phosphorylated at S62.
Bioworld Molecular Weight: 57 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Phospho-c-Myc(S62) on different lysates using anti-Phospho-c-Myc(S62) antibody at 1/1,000 dilution.
Positive control:
Lane 1: A549
Lane 2: HCT116
Lane 3: Hela
Lane 4: HepG2, ICC staining Phospho-c-Myc(S62) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: bHLHe39, MYC, AU016757, Avian myelocytomatosis viral oncogene homolog, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39, MRTL, Myc, Myc protein, Myc proto oncogene protein, Myc proto-oncogene protein, myc-related translation/localization regulatory factor, MYC, Myc2, MYCC, Myelocytomatosis oncogene, Niard, Nird, Oncogene Myc, OTTHUMP00000158589, Proto-oncogene c-Myc, Protooncogene homologous to myelocytomatosis virus, RNCMYC, Transcription factor p64, Transcriptional regulator Myc-A, V-Myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian),
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser62 of human c-Myc.
Conjugate: Unconjugated
Modification: Phosphorylation