Product Description

c-Myc Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79653-50, BS79653-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P01106

Host: Rabbit

Reactivity: Mouse, Rat

Applications: IHC

All Applications: IHC,1:50 - 1:100

Background: The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Immunogen: A synthetic peptide of human c-Myc(NP_002458.2).

Conjugate: Unconjugated

Modification: Unmodified

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry of paraffin-embedded mouse liver using c-Myc antibody at dilution of 1:100.Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded rat heart using c-Myc antibody at dilution of 1:100.Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.