C4BPB Polyclonal Antibody-BS8157

C4BPB Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS8157-50, BS8157-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P20851

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:2000
IHC: 1:50~1:200
IF: 1:50~1:100

Background: Complement component 4-binding protein (C4BP) is a plasma glycoprotein that inhibits the classical pathway of complement activation, which is mediated through antibody targeting of foreign antigen. Structurally, C4BP is a disulfide linked, multimeric protein that is composed of seven α chains and one β chain. C4BP functions as a cofactor for C3β inactivator in the cleavage of C3β, and accelerates the decay of C4βC2α (C3 convertase) by acting as a cofactor in the cleavage of C4β by factor I. Streptococcal strains that express Ig-binding cell surface molecules, which are members of the M protein family,can bind to overlapping C4β binding sites in C4BP and therefore, interfere with the classical pathway of complement activation. Bacteria-bound C4BP may be an evolved mechanism that downregulates complement activation in the bacterial host microenvironment, thereby reducing the occurances of bacterial opsonization and phagocytosis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: C4BPB polyclonal antibody detects endogenous levels of C4BPB protein.

Bioworld Molecular Weight: ~ 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of C4BPB polyclonal antibody at 1:500 dilution
Lane A: HEK293T whole cell lysate (30ug)
Lane B: H1688 whole cell lysate (30ug)
Lane C: MCF-7 whole cell lysate (30ug)
Lane D: The Liver tissue lysate of Mouse (30ug)
Lane E: The Liver tissue lysate of Rat (30ug), Immunohistochemical analysis of C4BP beta staining in human colorectal cancer formalin fixed paraffin embedded tissue section., Immunofluorescent analysis of C4BP beta staining in HEK293T cells.

Alternative Name: C4b-binding protein beta chain; C4BPB

Immunogen: Synthetic peptide, corresponding to Human C4BPB.

Conjugate: Unconjugated

Modification: Unmodified