CA II (N229) Polyclonal Antibody-BS3440

CA II (N229) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3440-50, BS3440-100

Sizes: 50µl, 100µl

Swiss-Prot: P00918

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes including respiration, calcification, acid-base balance and bone resorption, as well as the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. The human CA2 gene, which maps to chromosome 8q21, encodes CA II, a cytoplasmic protein that has the highest turnover rate and widest tissue distribution of any known human CA isozyme.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: CA II (N229) polyclonal antibody detects endogenous levels of CA II protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CA II (N229) pAb at 1:500 dilution
Lane 1: HCT116 whole cell lysate (40ug)
Lane 2: The Kidney tissue lysate of Mouse (40ug)
Lane 3: The Brain tissue lysate of Rat (20ug)
Lane 4: HEK293T whole cell lysate (10ug), Immunohistochemistry (IHC) analyzes of CA II (N229) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 28 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Carbonic anhydrase 2; Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II; CA2

Immunogen: Synthetic peptide, corresponding to amino acids 201-250 of Human CA II.

Conjugate: Unconjugated

Modification: Unmodified