Product Description

CA IX Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90159-50, BS90159-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q16790(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

All Applications: WB:1:1,000-1:2,000
IHC:1:50-1:200

Background: Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes including respiration, calcification, acid-base balance and bone resorption, as well as the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. The human CA2 gene, which maps to chromosome 8q21, encodes CA II, a cytoplasmic protein that has the highest turnover rate and widest tissue distribution of any known human CA isozyme. The human CA4 gene, which maps to chromosome 17q23, encodes CA IV, a membrane-anchored isozyme that is expressed on the luminal surfaces of pulmonary capillaries and proximal renal tubules. The human CA9, CA12 and CA14 genes, which map to chromosomes 9p13, 15q22 and 1q21, respectively, encode transmembrane proteins that have unique patterns of tissue-specific expression. CA IX is specifically expressed in clear-cell renal carcinomas, whereas CA XII is highly expressed in normal tissues, such as kidney, colon and pancreas. Human CA XIV is also expressed in normal tissues, such as brain, but differs from CA XII in its expression pattern.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: CA IX polyclonal antibody detects endogenous levels of CA IX protein.

Bioworld Molecular Weight: 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Carbonic anhydrase 9 on human lung lysates using anti- Carbonic anhydrase 9 antibody at 1/1,000 dilution., Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti- Carbonic anhydrase 9 antibody. Counter stained with hematoxylin.

Alternative Name: CA-IX, CAIX, RCC-associated antigen G250, CA9, CA-IX, CA9, CAH9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, Carbonic anhydrase IX, Carbonic dehydratase, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC associated protein G250, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified