Product Description

Cadherin pan Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66108-50, MB66108-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellμlar domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development. The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellμlar structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton. While β- and γ-catenin play structural roles in the junctional complex, p120 regμlates cadherin adhesive activity and trafficking. Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers. Research studies indicate that cancer cells have upregμlated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellμlar invasion. Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascμlar permeability and tumor angiogenesis. Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IHC

Alternate Names: CDH1, CDHE, UVO, Cadherin-1, CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorμlin, CD324, CDH2, CDHN, NCAD, Cadherin-2, CDw325, Neural cadherin, N-cadherin, CD325, CDH3, CDHP, Cadherin-3, Placental cadherin, P-cadherin, CDH4, Cadherin-4, Retinal cadherin, R-CAD, R-cadherin

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Cadherin pan. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IHC(1/100 - 1/300)

Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of Cadherin pan protein.

Molecular Weight: ~ 125 kDa

Pathway: Adherens Junction signaling, ErbB HER Signaling, Wntβ catenin signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P12830, P19022, P22223, P55283

Product: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Research Use Only