CALCA Polyclonal Antibody
Catalogue Numbers: BS60494-50, BS60494-100
Sizes: 50µl, 100µl
Alternative Name: Calcitonin gene-related peptide 1; Alpha-type CGRP; Calcitonin gene-related peptide I; CGRP-I; CALCA; CALC1
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P06881/P01258
Host: Rabbit
Reactivity: Human, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: Calcitonin is a 32 amino acid polypeptide hormone that preserves skeletal integrity and reduces blood calcium levels by decreasing osteoclast activity in bones, calcium and phosphate reabsorption by kidney tubules and calcium absorption by the intestines. The secretion of Calcitonin from the thyroid is regulated in part by estrogen, which increases Calcitonin mRNA levels. The Calcitonin gene, CALCA, undergoes tissue-specific RNA alternative splicing, resulting in the production of different mRNA transcripts. One transcript encodes procalcitonin as well as both calcium-lowering processed active polypeptides, Calcitonin and katacalcin. An alternative transcript of CALCA encodes the precursor for the neuropeptide referred to as Calcitonin gene-related peptide 1, also designated CGRP1 or å-CGRP. CGRP is a widely distributed vasodilatory peptide. Calcitonin and katacalcin are produced primarily in the thyroid, while CGRP is produced in neuronal cells. A second CGRP related gene, CALCB, thought to be derived from an gene duplication event, has been identified in mouse, rat and human. Unlike CALCA, CALCB is not subject to alternative splicing and encodes a single transcript designated CGRP2 or β-CGRP. Mature CGRP1 and CGRP2 share significant sequence identity at the protein level differing by only 1-3 amino acid residues, depending on the species.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: CALCA polyclonal antibody detects endogenous levels of CALCA protein.
Bioworld Molecular Weight: ~ 13 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of CALCA polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate
Lane 2: H9C2 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human CALCA.
Conjugate: Unconjugated
Modification: Unmodified