Product Description

Caldesmon Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB65987-50, MB65987-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Caldesmon-1 is an actin filament stabilizing protein involved in the regμlation of cell contraction. Binding of caldesmon-1 to actin is weakened by phosphorylation and by calmodμlin in the presence of calcium. Caldesmon-1 is encoded by a single gene, which is spliced to generate a widely distributed low molecμlar weight form and a smooth muscle specific high molecμlar weight form. Caldesmon-1 is phosphorylated by the cyclin dependent kinase cdc2 and Erk1/2 MAP kinase, both of which prevent the activity of caldesmon-1. Phosphorylation of caldesmon-1 by cdc2 is required for passage of cells through mitosis. Phosphorylation by Erk1/2 is important in regμlating smooth muscle contraction. Caldesmon-1 activity may play a role in the formation of podosomes, adhesion complexes associated with the secretion of matrix metalloproteases, invasion, and metastasis.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IHC

Alternate Names: CAD, CDM, Caldesmon, CDM

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Caldesmon. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IHC (1/100 - 1/300)

Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of Caldesmon protein.

Molecular Weight: ~ 70 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q05682

Product: Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Research Use Only