Product Description

Caldesmon Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9915M-50, BS9915M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q05682

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB: 1:1000-1:2000
IHC/ICC/IF: 1:50-1:200
FC: 1:50-1:100

Background: Caldesmon, Filamin 1, Nebulin and Villin are differentially expressed and regulated Actin binding proteins. Both muscular and non-muscular forms of Caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and Myosin. Alternative splicing of the gene encoding Caldesmon results in five isoforms. Muscular Caldesmon (isoform 1), also designated high molecular weight Caldesmon or H-Caldesmon (H-CAD), is expressed predominantly on thin filaments in smooth muscle. Non-muscular Caldesmon (isoforms 2-5), also designated low molecular weight Caldesmon or L-Caldesmon (L-CAD), is widely expressed in non-muscle tissues and cells.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of Caldesmon and does not cross-react with related proteins.

Bioworld Molecular Weight: 70-80 kDa non-muscle
120-150 kDa smooth muscle

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Caldesmon Rabbit mAb at 1:1000 dilution
Lane 1: NIH/3T3 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caldesmon antibody.Counter stained with hematoxylin., ICC staining Caldesmon in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Caldesmon; CDM; CALD1; CAD; CDM

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified