Product Description

Caldesmon (S783) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1437-50, BS1437-100

Sizes: 50µl, 100µl

Swiss-Prot: Q05682

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: Caldesmon, Filamin 1, Nebulin and Villin are differentially expressed and regulated Actin binding proteins. Both muscular and non-muscular forms of Caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and Myosin. Alternative splicing of the gene encoding Caldesmon results in five isoforms. Muscular Caldesmon (isoform 1), also designated high molecular weight Caldesmon or H-Caldesmon (H-CAD), is expressed predominantly on thin filaments in smooth muscle. Non-muscular Caldesmon (isoforms 2-5), also designated low molecular weight Caldesmon or L-Caldesmon (L-CAD), is widely expressed in non-muscle tissues and cells.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Caldesmon (S783) polyclonal antibody detects endogenous levels of Caldesmon protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Caldesmon (S783) polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 whole cell lysate (20ug)
Lane 2: Hela whole cell lysate (40ug)
Lane 3: A549 whole cell lysate (40ug)
Lane 4: PC12 whole cell lysate (40ug)
Lane 5: MEF whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Caldesmon (S783) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: 70-80 kDa non-muscle
120-150 kDa smooth muscle

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Caldesmon; CDM; CALD1; CAD; CDM

Immunogen: Synthetic peptide, corresponding to amino acids 750-800 of Human Caldesmon.

Conjugate: Unconjugated

Modification: Unmodified