Product Description

Caldesmon(Ab-759)Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65240-50, BS65240-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Swiss-Prot: Q05682

Host: Mouse/IgG2b, Kappa

Reactivity: Human

Applications: IHC-p, IF (paraffin section)

All Applications: IHC-p 1:100-500，WB 1:500-2000

Background: caldesmon 1(CALD1) Homo sapiens This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008],

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of human Caldesmon. Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section

Immunogen: Synthesized peptide derived from human Caldesmon

Conjugate: Unconjugated

Modification: Unmodified

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemical analysis of paraffin-embedded Liomyoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min)., Immunohistochemical analysis of paraffin-embedded Colon. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min)., Western blot analysis of CaldesmonAntibody at 1:1000 dilution.