Product Description

Calmodulin (K75) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2989-50, BS2989-100

Sizes: 50µl, 100µl

Swiss-Prot: P62158

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: The level of intracellular calcium is tightly regulated in all eukaryotic cells. A modest increase in this level can result in a myriad of physiological responses, most of which are mediated by calmodulin (CaM), the universal calcium sensor. CaM directly modulates the activity of protein kinases and phosphatases, ion channels and nitric oxide synthetases. It is generally involved in such diverse processes as cell proliferation, endocytosis, cellular adhesion, protein turn over and smooth muscle contraction. CaM (calmodulin) is an acidic protein, 148 amino acids in length, with four helix-loop-helix calcium binding domains.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Calmodulin (K75) polyclonal antibody detects endogenous levels of Calmodulin protein

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Calmodulin (K75) pAb at 1:500 dilution
Lane 1: Hela whole cell lysate (40ug)
Lane 2: PC3 whole cell lysate (40ug)
Lane 3: C6 whole cell lysate (40ug)
Lane 4: BV2 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Calmodulin (K75) pAb in paraffin-embedded human brain and breast carcinoma tissue.

Bioworld Molecular Weight: ~ 22 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Calmodulin; CaM; CALM1; CALM; CAM; CAM1; CALM2; CAM2; CAMB; CALM3; CALML2; CAM3; CAMC; CAMIII

Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human Calmodulin.

Conjugate: Unconjugated

Modification: Unmodified