Product Description

Calpain 1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9107-50, MB9107-100

Product: Mouse IgM, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P07384(Human) O35350(Mouse) P97571(Rat)

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: ICC, IHC, FC

All Applications: ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Calpain 1, also designated µ-calpain, is an intracellular calcium-dependent protease that cleaves cyto-skeletal and submembranous proteins. Calpains are nonlysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2+-dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpain-1 co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction.

Purification and Purity: ProG purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: Calpain 1 monoclonal antibody detects endogenous levels of Calpain 1 protein.

Bioworld Molecular Weight: 82 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining Calpain 1(green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin.

Alternative Name: CANP 1, muCANP, CAPN1, CANPL1, PIG30, Ca2 activated neutral protease, Calcium activated neutral proteinase, Calcium activated neutral proteinase small subunit, Calcium dependent protease small subunit 1, Calcium dependent protease small subunit, Calcium-activated neutral proteinase 1, Calpain 1, Calpain 1 large subunit, Calpain 1, (mu/I) large subunit, Calpain mu type, Calpain mu-type, Calpain regulatory subunit, Calpain small subunit 1, Calpain, large polypeptide L1, Calpain-1 catalytic subunit, Calpain-1 large subunit, CAN1, CANP 1, CANP, CANP small subunit, CANP1, CANPL 1, CANPL1, CAPN 1, CAPN1, Cell proliferation inducing protein 30, Cell proliferation-inducing gene 30 protein, Micromolar Calpain, Micromolar-calpain, Mu Calpain, muCANP, muCL,

Immunogen: Recombinant protein.

Conjugate: Unconjugated

Modification: Unmodified