Calpain 2 (K354) Polyclonal Antibody-BS3696

Calpain 2 (K354) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3696-50, BS3696-100

Sizes: 50µl, 100µl

Swiss-Prot: P17655

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Mu Calpain, and m calpain, also known as Calpain 2, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. Both Calpains 1 and 2 are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Calpain 2 (K354) polyclonal antibody detects endogenous levels of Calpain-2 catalytic subunit protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Calpain 2 (K354) pAb at 1:500 dilution
Lane 1: Beas-2B whole cell lysate (40ug)
Lane 2: HEK293T whole cell lysate (40ug)
Lane 3: PC3 whole cell lysate (40ug)
Lane 4: The Kidney tissue lysate of Mouse (40ug)
Lane 5: The Kidney tissue lysate of Rat (40ug), Immunohistochemistry (IHC) analyzes of Calpain 2 (K354) pAb in paraffin-embedded human brain tissue.

Bioworld Molecular Weight: ~ 80 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Calpain-2 catalytic subunit; Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; Calpain large polypeptide L2; Calpain-2 large subunit; Millimolar-calpain; M-calpain; CANPL2; CAPN2

Immunogen: Synthetic peptide, corresponding to amino acids 320-370 of Human Calpain 2.

Conjugate: Unconjugated

Modification: Unmodified