Product Description

Calpain 2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90172-50, BS90172-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P17655(Human) O08529(Mouse) Q07009(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1:2,000
IHC:1:50-1:200

Background: Calpain, an intracellular calcium-dependent protease that cleaves cytoskeletal and submembranous proteins, is thought to play a role in cytoskeletal reorganization and muscle protein degradation. Calpain exists as a heterodimer composed of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpastatin regulates calpain by inhibiting both the proteolytic activity of calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Calpain 2 polyclonal antibody detects endogenous levels of Calpain 2 protein.

Bioworld Molecular Weight: 80 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Calpain 2 on Hela cell (1) and rat skin tissue (2) lysate using anti-Calpain 2 antibody at 1/1,000 dilution., ICC staining Calpain 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CANP 2, M-calpain, CAPN2, CANPL2, Calcium activated neutral proteinase 2, Calcium activated neutral proteinase, Calcium-activated neutral protease 2, catalytic subunit, Calcium-activated neutral proteinase 2, CALP80, Calpain 2 (m/II) large subunit, Calpain 2 catalytic subunit, Calpain 2 large catalytic subunit, Calpain 2 large subunit, Calpain 2, large [catalytic] subunit, Calpain large polypeptide L2, Calpain M type, Calpain M-type, Calpain-2 catalytic subunit, Calpain-2 large subunit, Calpain2, CAN2, CANP 2, CANP L2, CANP2, CANPL 2, CANPL2, CANPml, Capa2, CAPN 2, CAPN2, FLJ39928, M calpain, M calpin, M type, M-calpain, mCANP, Millimolar calpain, Millimolar-calpain,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified