Product Description

Calpain 2 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: CAS7518-50, CAS7518-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Calpain is a calcium-dependent thiol proteinase that is functionally active as a heterodimer composed of a small regμlatory subunit and one of at least two large catalytic subunits (calpain 1 or calpain 2). In vitro, calpain 1 (mu-calpain) requires micromolar levels of calcium, while calpain 2 (M-calpain) requires millimolar levels of calcium for activation. The regμlation of calpain in vivo is the subject of many current studies, which suggest that proteolytic activity is regμlated post-transcriptionally by mechanisms such as calcium requirements, subcellμlar localization of the heterodimer, phosphorylation via the EGFR-Erk signaling cascade, endogenous inhibitors (calpastatin) and autoproteolytic cleavage. Calpastatin negatively regμlates autoproteolytic cleavage of calpain 1 between Gly27 and Leu28. Calpain influences cell migration by modifying rather than degrading its substrates responsible for cell adhesion and cytoskeletal arrangement. Control of calpain activity has caught the attention of drug development since limiting its activity coμld mute invasiveness of tumors or chronic inflammation.

Category: Primary Antibody

Reactivity: Human, Rat, Mouse

Host: Rabbit

Applications: WB, IP

Alternate Names: Calpain-2 catalytic subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, Calpain large polypeptide L2, Calpain-2 large subunit, Millimolar-calpain, M-calpain, CAPN2, CANPL2

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant protein of human Calpain 2.

Dilution: WB: 1:5000~1:10000
IP:1:2000~1:8000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE).

Specificity: Calpain 2 polyclonal antibody detects endogenous levels of Calpain 2 protein.

Molecular Weight: ~ 80 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P17655

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Research Use Only