Product Description

Calpain 2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: CAS7518-50, CAS7518-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P17655

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB, IP,

All Applications: WB: 1:5000~1:10000
IP:1:2000~1:8000

Background: Calpain is a calcium-dependent thiol proteinase that is functionally active as a heterodimer composed of a small regulatory subunit and one of at least two large catalytic subunits (calpain 1 or calpain 2). In vitro, calpain 1 (mu-calpain) requires micromolar levels of calcium, while calpain 2 (M-calpain) requires millimolar levels of calcium for activation. The regulation of calpain in vivo is the subject of many current studies, which suggest that proteolytic activity is regulated post-transcriptionally by mechanisms such as calcium requirements, subcellular localization of the heterodimer, phosphorylation via the EGFR-Erk signaling cascade, endogenous inhibitors (calpastatin) and autoproteolytic cleavage. Calpastatin negatively regulates autoproteolytic cleavage of calpain 1 between Gly27 and Leu28. Calpain influences cell migration by modifying rather than degrading its substrates responsible for cell adhesion and cytoskeletal arrangement. Control of calpain activity has caught the attention of drug development since limiting its activity could mute invasiveness of tumors or chronic inflammation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Calpain 2 polyclonal antibody detects endogenous levels of Calpain 2 protein.

Bioworld Molecular Weight: ~ 80 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Calpain 2 polyclonal antibody at 1:5000 dilution
Lane 1: Hela whole cell lysate (30ug)
Lane 2: A375 whole cell lysate (30ug)
Lane 3: The Skin tissue lysate of Rat (40ug)
Lane 4: SGC7901 whole cell lysate (40ug)
Lane 5: P12 whole cell lysate (40ug)
Lane 6: AML-12 whole cell lysate (40ug), Immunoprecipitation - Calpain 2 Polyclonal Antibody

Alternative Name: Calpain-2 catalytic subunit; Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; Calpain large polypeptide L2; Calpain-2 large subunit; Millimolar-calpain; M-calpain; CAPN2; CANPL2

Immunogen: Recombinant protein of human Calpain 2.

Conjugate: Unconjugated

Modification: Unmodified