Product Description

Calponin 2 (D156) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3097-50, BS3097-100

Sizes: 50µl, 100µl

Swiss-Prot: Q99439

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC, IF

Background: Calponin, a 34 kDa protein, regulates smooth muscle cell contraction and is a marker of smooth muscle cell differentiation. Calponin, an actin- and tropomyosin-binding protein, is characterized as an inhibitory factor of smooth-muscle actomyosin activity. Calponin is implicated in the regulation of smooth muscle contraction through its interaction with F-actin and inhibition of the actin-activated MgATPase activity of phosphorylated myosin.Both properties are lost following phosphorylation (primarily at serine 175) by protein kinase C or calmodulin-dependent protein kinase II. The three forms of Calponin, Calponin 1 (basic Calponin), Calponin 2 (neutral Calponin), and Calponin 3 (acidic Calponin) are found in smooth muscle tissue. Additionally, Calponin 2 is found in heart muscle tissue and Calponin 3 is found in the brain.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Calponin 2 (D156) polyclonal antibody detects endogenous levels of Calponin 2 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Calponin 2 (D156) pAb at 1:500 dilution
Lane 1: AML-12 whole cell lysate (40ug)
Lane 2: H9C2 whole cell lysate (20ug)
Lane 3: L02 whole cell lysate (40ug)
Lane 4: HepG2 whole cell lysate (40ug)
Lane 5: Hela whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Calponin 2 (D156) pAb in paraffin-embedded human brain tissue.

Bioworld Molecular Weight: ~ 33 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Calponin-2; Calponin H2, smooth muscle; Neutral calponin; CNN2

Immunogen: Synthetic peptide, corresponding to amino acids 122-170 of Human Calponin 2.

Conjugate: Unconjugated

Modification: Unmodified