Calreticulin Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB9108-50, MB9108-100
Product: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P27797(Human)
Host: Mouse
Reactivity: Human
Applications: WB, IHC ICC FC
All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: Calnexin and calregulin (also called calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum, Calnexin to the membrane and calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor calregulin contains the calcium-binding "E-F hand" motif found in calmodulins. Calnexin and calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins.
Purification and Purity: ProG affinity purified.
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: Calreticulin monoclonal antibody detects endogenous levels of Calreticulin protein.
Bioworld Molecular Weight: 55 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Calreticulin on HL-60 (1) and A431 (2) cell lysates using anti-Calreticulin antibody at 1/1,000 dilution., ICC staining Calreticulin (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: ERp60, CALR, CRTC, Autoantigen RO, CALR, CALR protein, CALR, Calregulin, Calreticulin, cC1qR, CRP55, CRT, CRTC, Endoplasmic reticulum resident protein 60, Epididymis secretory sperm binding protein Li 99n, ERp60, FLJ26680, grp60, HACBP, HEL S 99n, RO, Sicca syndrome antigen A (autoantigen Ro; calreticulin), Sicca syndrome antigen A, SSA,
Immunogen: Recombinant protein.
Conjugate: Unconjugated
Modification: Unmodified