Product Description

Calreticulin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90173-50, BS90173-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P27797(Human) P14211(Mouse) P18418(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: Calnexin and calregulin (also called calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum, Calnexin to the membrane and calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor calregulin contains the calcium-binding "E-F hand" motif found in calmodulins. Calnexin and calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Calreticulin polyclonal antibody detects endogenous levels of Calreticulin protein.

Bioworld Molecular Weight: 60 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Calreticulin on different lysates using anti-Calreticulin antibody at 1/1,000 dilution.
Positive control:
Lane 1: SH-SY-5Y
Lane 2: HL-60, ICC staining Calreticulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: ERp60, CALR, Autoantigen RO, CALR, CALR protein, CALR, Calregulin, Calreticulin, cC1qR, CRP55, CRT, CRTC, Endoplasmic reticulum resident protein 60, Epididymis secretory sperm binding protein Li 99n, ERp60, FLJ26680, grp60, HACBP, HEL S 99n, RO, Sicca syndrome antigen A (autoantigen Ro; calreticulin), Sicca syndrome antigen A, SSA,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified