Product Description

CaMKIIα (phospho-T286) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS5009-50, BS5009-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The Ca2+/calmodμlin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is an ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+ and calmodμlin (CaM) and has been implicated in regμlation of the cell cycle and transcription. There are four CaMKII isozymes, designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIIα is autophosphorylated on Thr 286 upon the binding of the Ca2+/CaM complex to the autoinhibitory domain of CaMKII. This process is called Ca2+/CaM trapping, which is thought to be involved in the synaptic encoding of information.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: Calcium/calmodμlin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha, CAMK2A, CAMKA, KIAA0968, CaMK2-a, CaMK2a, CaMK-2a

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human CaMKIIα around the phosphorylation site of Threonine 286.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-CaMK2-a (T286) polyclonal antibody detects endogenous levels of CaMK2-a protein only when phosphorylated at Thr286.

Molecular Weight: ~ 54 kDa

Pathway: Phospholipase Signaling, Regμlation of Microtubμle Dynamics, G Protein-coupled Receptors Signaling to MAPK Erk, ErbB HER Signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9UQM7

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only