Product Description

CaMKII alpha/delta Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16426-50, BZ16426-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: Q9UQM7/Q13557

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Background: The product of CAMK2A (calcium/calmodulin dependent protein kinase II alpha) belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for CAMK2A.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 54 kDa; Observed MW: 54 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CaMKII alpha/delta in NIH3T3/K562 lysates using CaMKII alpha/delta antibody., Immunofluorescence analysis of CaMKII alpha/delta in MCF-7 using CaMK2 alpha/delta antibody. The picture on the right is blocked using the synthesized peptide., Immunohistochemistry analysis of paraffin-embedded Human brain tissue using CaMK2 alpha/delta antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Sample with blocking peptide on the right., Western blot analysis of CaMKII alpha/delta in NIH/3T3 and K562 lysates using CaMKII alpha/delta antibody. The Lane on the right is blocked with the synthesized peptide.

Alternative Name: CAMK2A; CAMKA; KIAA0968; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMK2D; CAMKD; Calcium/calmodulin-dependent protein kinase type II subunit delta; CaM kinase II

Immunogen: The antiserum was produced against synthesized peptide derived from human CaMK2 alpha/delta. AA range:256-305

Conjugate: Unconjugated

Modification: Unmodified