Product Description

Caper (R199) Polyclonal Antibody

Catalogue Numbers: BS1891-50, BS1891-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75787

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Caper, also known as splicing factor HCC1 or hepatocellular carcinoma protein 1 and RNA binding region containing protein 2 (RNPC2), acts as a transcriptional coactivator for steroid nuclear receptors c-Jun, ERα and ERβ. Caper, a nuclear protein with highest concentrations in nuclear speckles, plays a role in the pre-mRNA splicing process. Two isoforms of Caper, HCC1.3 and HCC1.4, co-localize with pre-mRNA splicing factor SC35 and uridine-rich small nuclear RNAs. Caper is a widely expressed protein with highest levels detected in skeletal muscle, lung, brain and pancreas.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Caper (R199) pAb detects endogenous levels of Caper (R199) protein.

Extra Notes: Western blot (WB) analysis of Caper (R199) pAb at 1:500 dilution
Lane1:C6 whole cell lysate(40ug)
Lane2:3T3-L1 whole cell lysate(40ug)
Lane3:HepG2 whole cell lysate(40ug)
Lane4:U-87MG whole cell lysate(40ug)
Lane5:HEK293T whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 39 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.2; Histone H3/m; Histone H3/o; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.3; 3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3C; Histone H3.5; H3F3C

Immunogen: Synthetic methylpeptide derived from human Histone H3 around the methylation site of Lysine 80.

Conjugate: Unconjugated

Modification: Unmodified