CAPG Polyclonal Antibody-AP6017

CAPG Polyclonal Antibody

Catalogue Numbers: AP6017-50, AP6017-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P40121

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Caldesmon, Filamin 1, Nebulin, Plastin, ADF, Gelsolin, CapG, Dematin and Cofilin are differentially expressed Actin-binding proteins. Both muscular (CDh) and non-muscular (CD1) forms of Caldesmon bind to Actin as well as to Calmodulin and Myosin. CDh is expressed predominantly on thin filaments in smooth muscle, whereas CD1 is widely expressed in non-muscle tissues and cells. CapG, also designated Actin-regulatory protein and macrophage-capping protein, is a macrophage-specific protein that reversibly blocks the barbed ends of Actin filaments, but does not sever preformed ones. The interactions of CapG with Actin may be important in the regulation of nuclear and cytoplasmic structures. CapG is a calcium-sensitive DNA-binding protein that plays a role in macrophage function. It is expressed in macrophages and macrophage-like cells and can localize both to the nucleus and the cytoplasm.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CAPG polyclonal antibody detects endogenous levels of CAPG protein.

Extra Notes: Western blot (WB) analysis of CAPG pAb at 1:500 dilution
Lane1:The Lung tissue lysate of Mouse(40ug)
Lane2:The Lung tissue lysate of Rat(40ug)
Lane3:A549 whole cell lysate(40ug)
Lane4:H1792 whole cell lysate(40ug)
Lane5:A375 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 43 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Macrophage-capping protein; Actin regulatory protein CAP-G; CAPG; AFCP, MCP

Immunogen: Recombinant fragment corresponding to a region within amino acids 62-349 of Human CAPG.

Conjugate: Unconjugated

Modification: Unmodified