Cardiac Troponin I (TNNI3) Polyclonal Antibody-BS74248

Cardiac Troponin I (TNNI3) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74248-50, BS74248-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P19429

Host: Rabbit

Reactivity: Mouse, Rat

Applications: WB, IF/ICC, IP,

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 | IP,1:50 - 1:100

Background: Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 30kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Cardiac Troponin I antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s., Immunofluorescence analysis of mouse heart cells using Cardiac Troponin I antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 600ug extracts of Mouse heart cells using 3ug Cardiac Troponin I antibody. Western blot was performed from the immunoprecipitate using Cardiac Troponin I antibody at a dilution of 1:1000.

Alternative Name: TNNI3; CMD1FF; CMD2A; CMH7; RCM1; TNNC1; cTnI; troponin I3

Immunogen: Recombinant fusion protein of human Cardiac Troponin I (TNNI3)(NP_000354.4).

Conjugate: Unconjugated

Modification: Unmodified