Cardiac troponin T (TNNT2) Polyclonal Antibody-BS6013

Cardiac troponin T (TNNT2) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6013-50, BS6013-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P45379

Host: Rabbit

Reactivity: Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:100 - 1:200 | IF/ICC,1:50 - 1:200

Background: The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 42KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Cardiac troponin T antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunofluorescence analysis of rat heart cells using Cardiac troponin T Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of mouse heart cells using Cardiac troponin T Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: TNNT2; CMD1D; CMH2; CMPD2; LVNC6; RCM3; TnTC; cTnT; troponin T2

Immunogen: Recombinant fusion protein of human Cardiac troponin T (Cardiac troponin T (TNNT2))(NP_001001431.1).

Conjugate: Unconjugated

Modification: Unmodified