Product Description

CARP Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS71931-50, BS71931-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15327

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB 1:500 - 1:2000

Background: A proposed genetic marker of cardiac hypertrophy, CARP (cardiac ankyrin repeat protein, ANKRD1, Ankyrin repeat domain-containing protein 1,) is a nuclear protein with an established role in regulation of cardiac gene expression. A distinct increase in CARP expression occurs in rats with abdominal aorta constriction, spontaneous hypertension and Dahl salt-sensitivity. In cardiomyocytes, CARP inhibits transcription of both cardiac troponin C and atrial natriuretic factor. Specifically, expression of the CARP gene, which lies downstream of the cardiac homeobox gene Nkx2.5, inhibits Nkx2.5 transactivation of atrial natriuretic factor promoter. An increase in CARP expression is observed in the ventricular tissue of patients with endstage heart failure.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CARP polyclonal antibody detects endogenous levels of CARP protein.

Bioworld Molecular Weight: 36kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot analysis of extracts of various cell lines, using ANKRD1 antibody at 1:1000 dilution.

Alternative Name: Ankyrin repeat domain-containing protein 1; Cardiac ankyrin repeat protein; Cytokine-inducible gene C-193 protein; Cytokine-inducible nuclear protein; ANKRD1; C193; CARP; HA1A2

Immunogen: Recombinant protein of human ANKRD1

Conjugate: Unconjugated

Modification: Unmodified