Product Description

CARS Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6497-50, BS6497-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P49589

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Background: Aminoacyl-tRNA synthetases consist of a family of enzymes that catalyze the specific aminoacylation of tRNA by their cognate amino acid in the initial step of ribosome-dependent protein biosynthesis. Cytoplasmic CysRS (Cysteinyl-tRNA synthetase, cytoplasmic), also known as CARS, is a 748 amino acid member of the class-I aminoacyl-tRNA synthetase protein family. Cytoplasmic CysRS is a monomeric protein that binds one zinc ion per subunit for use as a cofactor. Cytoplasmic CysRS uses ATP to convert L-cysteine and tRNA(Cys) into ADP, a diphosphate and L-cysteinyl-tRNA(Cys). A chromosomal aberration of the gene that encodes Cytoplasmic CysRS is associated with inflammatory myofibroblastic tumors (IMTs). Cytoplasmic CysRS is expressed as two isoforms produced by alternative splicing events.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CARS polyclonal antibody detects endogenous levels of CARS protein.

Bioworld Molecular Weight: ~ 85 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot (WB) analysis of CARS polyclonal antibody

Alternative Name: Cysteine--tRNA ligase, cytoplasmic; Cysteinyl-tRNA synthetase; CysRS; CARS

Immunogen: Recombinant full length Human CARS.

Conjugate: Unconjugated

Modification: Unmodified