CASP1 Polyclonal Antibody - E-AB-93260

CASP1 Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-93260-60, E-AB-93260-120, E-AB-93260-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: CASP1

Target Synonym: IC; ICE; Il1; Casp; Il1bc

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Mouse, Rat

Application: WB, IF

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: P29452

Background: Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B and interleukin 18 (IL18 as well as the pyroptosis inducer Gasdermin-D (GSDMD, into active mature peptides. Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a proinflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes. Cleaves a tetrapeptide after an Asp residue at position P1. Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD. In contrast to cleavage of interleukins IL1B and IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT part. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive. In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly (By similarity.

Concentration: 1mg/mL

Immunogen: A synthetic peptide of mouse CASP1

Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000, IF 1:50-1:200

Calculated MW: 45kDa

Observed MW: 48KDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only