Product Description

Caspase-1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6068-50, BS6068-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P29466

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 48KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Confocal immunofluorescence analysis of Raw264.7 cells using Caspase-1 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH/3T3 cells using Caspase-1 Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining., Immunofluorescence analysis of U2OS cells using Caspase-1 Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.

Alternative Name: CASP1; ICE; IL1BC; P45; caspase-1; Caspase 1

Immunogen: Recombinant fusion protein of human Caspase 1(NP_150635.1).

Conjugate: Unconjugated

Modification: Unmodified