Product Description

Caspase-1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90183-50, BS90183-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P29466(Human) P29452(Mouse) P43527(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC FC

All Applications: WB:1:1,000-1:2,000
FC:1:50-1:100
IHC:1:50-1:200

Background: Caspase-1, originally designated ICE (for IL-1 converting enzyme), is a member of the group of caspases with large prodomains. Caspase-1 promotes maturation of interleukin IL-1β and interleukin18 (IL-18) by proteolytic cleavage of precursor forms into biologically active pro-inflamatory cytokines. Active caspase-1, a (p20/p10)2 tetramer, is necessary and sufficient for cleavage of precursor IL-1 as well as for induction of apoptosis in some cell lines. The highly conserved family of caspases mediate many of the morphological and biochemical features of apoptosis, including structural dismantling of cell bodies and nuclei, fragmentation of genomic DNA, destruction of regulatory proteins and propagation of other pro-apoptotic molecules. The human Caspase-1 gene maps to chromosome 2q14 and encodes a cytoplasmic protein expressed in liver, heart, skeletal muscle kidney and testis. Caspase-1 has been implicated in inflammation, septic shock, and other situations such as wound healing and the growth of certain leukemias.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Caspase-1 polyclonal antibody detects endogenous levels of Caspase-1 protein.

Bioworld Molecular Weight: 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Caspase-1 on different lysates using anti-Caspase-1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: THP-1
Lane 2: Jurkat, Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Caspase-1 antibody. Counter stained with hematoxylin.

Alternative Name: CASP-1, IL-1BC, ICE, IL-1 beta-converting enzyme, CASP1, CASP-1, CASP1, CASP1, Caspase 1, Caspase-1 subunit p10, ICE, IL-1 beta-converting enzyme, IL-1BC, IL1 beta converting enzyme, IL1B convertase, Interleukin 1 beta convertase, Interleukin 1B converting enzyme, Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, p45,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified