Caspase-10 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90184-50, BS90184-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q92851(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, IHC, IP, , FC
All Applications: WB:1:1,000-1:2,000
FC:1:50-1:100
IHC:1:50-1:200
Background: Caspase-10, also designated Mch4, is recruited to the native TRAIL and CD9 death-inducing signaling complexes (DISCs) by the FADD/Mort1 adaptor protein complex. Caspase-10 requires the assembly of the FADD and DISC complexes for its recruitment and cleavage-induced activation during CD95-induced apoptosis of activated T cells. The N-terminus of caspase-10 contains FADD-like death effector domains further indicating that it associates with FADD to induce apoptosis. Caspase-10 is not required for apoptosis induction and when overexpressed, cannot reverse defects in apoptosis induction caused by caspase-8 deficiency. Granzyme B cleaves procaspase-10 at an IXXD-A processing sequence to produce mature caspase-10. Mutations in the caspase-10 gene in the prodomain, p17 large protease subunit and p12 small protease subunit have been linked to a number of non-Hodgkin lymphomas in humans.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Caspase-10 polyclonal antibody detects endogenous levels of Caspase-10 protein.
Bioworld Molecular Weight: 59 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Caspase-10 on K562 cells lysates using anti-Caspase-10 antibody at 1/1,000 dilution., Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Caspase-10 antibody. Counter stained with hematoxylin.
Alternative Name: CASP-10, FLICE2, CASP10, ALPS2, Apoptotic protease Mch-4, CASP 10, CASP-10, CASP10, CASPA, Caspase 10 apoptosis related cysteine peptidase, Caspase-10 subunit p12, FADD like ICE2, Fas associated death domain protein, FAS-associated death domain protein interleukin-1B-converting enzyme 2, FLICE 2, FLICE2, ICE like apoptotic protease 4, ICE-like apoptotic protease 4, Interleukin 1B converting enzyme 2, MCH 4,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified