Product Description

Caspase 2 (K152) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS2995-50, BS2995-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Caspase-2 (Nedd2, ICH-1) is an aspartate-specific cysteine protease that is activated in response to various apoptotic stimμli. Caspase-2 is unique among the caspases in that it has features of both upstream caspases (long prodomain) and downstream caspases (DEXD substrate specificity). Caspase-2 is highly expressed in the brain during development and is expressed at low levels in adμlt tissue. Specifically, caspase-2 localizes to the mitochondria, the Golgi, the cytoplasm and the nucleus. Caspase-2 exists as two isoforms, caspase-2L and caspase-2S, which are produced by alternative splicing and differ in their N- and C-termini. Caspase-2L acts as a positive regμlator of apoptosis, whereas caspase-2S functions as a negative regμlator of apoptosis. Following apoptotic stimμli, the caspase-2L precursor undergoes cleavage at Asp 153 to produce a fragment (p30). The p30 fragment undergoes further cleavage to generate a fragment containing amino acids 153-308 (p18) and a fragment containing amino acids 317-435 (p13 or p14). As apoptosis progresses, the p13 (p14) fragment can undergo further processing to yield a fragment containing amino acids 331-435 (p12).

Category: Primary Antibody

Reactivity: Human, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: Caspase-2, Caspase 2, Caspase2, CASP-2, Neural precursor cell expressed developmentally down-regμlated protein 2, NEDD-2, Protease ICH-1, CASP2, ICH1, NEDD2

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 140-180 of Human Caspase 2.

Dilution: WB: 1:500~1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Caspase 2 (K136) polyclonal antibody detects endogenous levels of Caspase 2 protein.

Molecular Weight: ~ 51 kDa

Pathway: Mitochondrial Control of Apoptosis,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P42575

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only