Caspase-2 Rabbit Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS9856M-50, BS9856M-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P42575
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, FC
All Applications: WB: 1:1000-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100
Background: Caspase-2 (Nedd2, ICH-1) is an aspartate-specific cysteine protease that is activated in response to various apoptotic stimuli. Caspase-2 is unique among the caspases in that it has features of both upstream caspases (long prodomain) and downstream caspases (DEXD substrate specificity). Caspase-2 is highly expressed in the brain during development and is expressed at low levels in adult tissue. Specifically, caspase-2 localizes to the mitochondria, the Golgi, the cytoplasm and the nucleus. Caspase-2 exists as two isoforms, caspase-2L and caspase-2S, which are produced by alternative splicing and differ in their N- and C-termini. Caspase-2L acts as a positive regulator of apoptosis, whereas caspase-2S functions as a negative regulator of apoptosis. Following apoptotic stimuli, the caspase-2L precursor undergoes cleavage at Asp 153 to produce a fragment (p30). The p30 fragment undergoes further cleavage to generate a fragment containing amino acids 153-308 (p18) and a fragment containing amino acids 317-435 (p13 or p14). As apoptosis progresses, the p13 (p14) fragment can undergo further processing to yield a fragment containing amino acids 331-435 (p12).
Purification and Purity: Protein A affinity purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: This antibody detects endogenous levels of Caspase-2 and does not cross-react with related proteins.
Bioworld Molecular Weight: ~ 48 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of Caspase-2 Rabbit mAb at 1:1000 dilution
Lane 1: 293 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Caspase-2 antibody.Counter stained with hematoxylin., ICC staining Caspase-2 in Hela cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
Alternative Name: Caspase-2; Caspase 2; Caspase2; CASP-2; Neural precursor cell expressed developmentally down-regulated protein 2; NEDD-2; Protease ICH-1; CASP2; ICH1; NEDD2
Immunogen: Recombinant antibody.
Conjugate: Unconjugated
Modification: Unmodified