Caspase 3 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB10448-50, MB10448-100
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Swiss-Prot: P42574
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IP,
All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
Background: Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 32 kDa; Observed MW: 32 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Caspase3 in K562, rat Brain, C6, 3T3, Hela lysates using Caspase 3 antibody., Immunohistochemistry analysis of paraffin-embedded Human tonsil using Caspase3 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.
Alternative Name: CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1
Immunogen: Recombinant protein of human Caspase-3
Conjugate: Unconjugated
Modification: Unmodified