Product Description

Caspase 3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90181-50, BS90181-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P42574(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, IHC, IP,

All Applications: WB:1:1,000-1:2,000
IHC:1:50-1:200

Background: Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Caspase 3 polyclonal antibody detects endogenous levels of Caspase 3 protein.

Bioworld Molecular Weight: 35 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of active+pro Caspase 3 on Jurkat cells lysates using anti-active+pro Caspase 3 antibody at 1/1,000 dilution., Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-active+pro Caspase 3 antibody. Counter stained with hematoxylin.

Alternative Name: CASP-3, CPP-32, SCA-1, CASP3, Apopain, CASP 3, CASP-3, CASP3, CASP3, Caspase 3, Caspase-3 subunit p12, CPP 32, CPP-32, CPP32B, Cysteine protease CPP32, PARP cleavage protease, Protein Yama, SCA-1, SCA1, SREBP cleavage activity 1, Yama,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified